Enzymatic Analysis of Organophosphorus Pesticides Using Cholinesterase Inhibition Activities

The effects of organophosphorus were examined with inhibition of the cholinesterase activity on tile chicken plasma in vivo and in vitro. The cholinesterase activity in chicken plasma determined by tile Ellman mettled was /min/g protein. After oral administration with 0.2 and 0.5 times of organophosphorus terbufos (1.81 mg/kg), cholinesterase activity were inhibited to 36% and 96% of control after 15min in vivo, respectively. After oral administration with 0.2 and 0.5 times of terbufos (1.81 mg/kg), then the recovery of cholinesterase activity followed to 99% and 56% of control after 11hr, respectively. Ki of phosphorodithioate and phosphorothioate with P=S was in vitro. Ki of phosphate and phosphorothiolate with P=O was . Toxicology of organophosphorus with P=S was higher than that of organophosphorus with P=S by oxidation. of phosphorodithioate and phosphorothioate with P=S was mg/L. of phosphate and phosphorothiolate with P=O was mg/L. Enzyme-Inhibition method with cholinesterase was the rapid bioassay method to detect the organohpophorus pesticides in vitro.