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Preparative Ultracentrifugation and Analytic Ultracentrifugation of Plasma Lipoproteins

Joseph Orr, Gerald L. Adamson, Frank T. Lindgren

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Abstract

There are several chapters and a book dealing with both preparative and analytical ultracentrifugation (AnUC) of plasma lipoproteins. However, what we would like to present are the procedures as currently done here at Donner Laboratory. They have been modified and improved since the earliest lipoprotein flotation was demonstrated in 1949, and this will present a combined, practical up-date of our chapter on AnUC in Blood Lipids and Lipoproteins (1972) and our chapter on preparative procedures in the American Oil Chemists' Society (AOCS) book, Analysis of Lipids and Lipoproteins (1975). This information is intended as a practical laboratory guide to anyone who wishes to do preparative and AnUC of plasma lipoproteins. 10 refs., 6 figs., 1 tab.

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What this paper is about

There are several chapters and a book dealing with both preparative and analytical ultracentrifugation (AnUC) of plasma lipoproteins. However, what we would like to present are the procedures as currently done here at Donner Laboratory. They have been modified and improved since the earliest lipoprotein flotation was demonstrated in 1949, and this will present a combined, practical up-date of our chapter on AnUC in Blood Lipids and Lipoproteins (1972) and our chapter on preparative procedures in the American Oil Chemists' Society (AOCS) book, Analysis of Lipids and Lipoproteins (1975). This information is intended as a practical laboratory guide to anyone who wishes to do preparative and AnUC of plasma lipoproteins. 10 refs., 6 figs., 1 tab.

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Available abstract

There are several chapters and a book dealing with both preparative and analytical ultracentrifugation (AnUC) of plasma lipoproteins. However, what we would like to present are the procedures as currently done here at Donner Laboratory. They have been modified and improved since the earliest lipoprotein flotation was demonstrated in 1949, and this will present a combined, practical up-date of our chapter on AnUC in Blood Lipids and Lipoproteins (1972) and our chapter on preparative procedures in the American Oil Chemists' Society (AOCS) book, Analysis of Lipids and Lipoproteins (1975). This information is intended as a practical laboratory guide to anyone who wishes to do preparative and AnUC of plasma lipoproteins. 10 refs., 6 figs., 1 tab.

Key concepts: Ultracentrifuge, Analytical Ultracentrifugation, Plasma lipoprotein, Chromatography, Chemistry, Biochemistry, Lipoprotein, Cholesterol

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