2013•DOAJ (DOAJ: Directory of Open Access Journals)Open access

Studies on genetic diversity in redgram genotypes using rapd markers.

K. C. Chandana, Lakkireddi Prasanthi, M. Reddi Sekhar, B.V. Bhaskara Reddy

Abstract

Genetic diversity was estimated among fifteen genotypes of redgram using 30 RAPD primers. A total of 172 RAPD amplicons were obtained; of which 114(66%) were polymorphic. The present polymorphism ranged from 25 to 100 per cent. A wide range (18.0 to 95.2%) of Jaccard's similarity coefficient was observed between the pairs of genotypes. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters. Among the 15 genotypes LRG-30 was found in separate cluster (cluster I), while all other genotypes grouped into other major cluster (cluster II) indicating a distinct background of this genotype

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Key concepts: RAPD, UPGMA, Jaccard index, Dendrogram, Biology, Genotype, Genetic diversity, Genetics