SSCP diversity of exon 2 of FSHβ gene in Huanghuai goat.
Yumin Yang, Ding JianPing, Zijun Zhang, Xiaorong Zhang, Hu MinLan, Zhang YuJun
Abstract
Yumin Yang, Ding JianPing, Zijun Zhang, Xiaorong Zhang, Hu MinLan, Zhang YuJun
Abstract
The polymorphism of exon 2 of FSHβ gene was studied by PCR-SSCP method in Huanghuai goat.The results showed that AA,AB and AC genotypes were detected in Huanghuai goats,and frequencies of them were 0.8514,0.1216 and 0.0270,respectively.Sequencing revealed C→T mutations at 47 bp and 147 bp in the AB genotype in comparison to the AA genotype,and C→T mutation at 147 bp and G→A mutation at 156 bp in the AC genotype in comparison to the AA genotype.
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The polymorphism of exon 2 of FSHβ gene was studied by PCR-SSCP method in Huanghuai goat.The results showed that AA,AB and AC genotypes were detected in Huanghuai goats,and frequencies of them were 0.8514,0.1216 and 0.0270,respectively.Sequencing revealed C→T mutations at 47 bp and 147 bp in the AB genotype in comparison to the AA genotype,and C→T mutation at 147 bp and G→A mutation at 156 bp in the AC genotype in comparison to the AA genotype.
Key concepts: Genotype, Exon, Single-strand conformation polymorphism, Biology, Genetics, Polymorphism (computer science), Molecular biology, Gene