2012Unpublished venueRequires access

Atlas of human pluripotent stem cells : derivation and culturing

Michal Amit, Joseph Itskovitz‐Eldor, Ilana Laevsky, Atara Novak

Open publisher page 13 citations

Abstract

Methods for the Derivation of Human Embryonic Stem Cell Lines.- Morphology of Human Embryonic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders.- Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder-Layer-Free Conditions.- Morphology of Undifferentiated Human Embryonic and Induced Stem Cells Grown in Suspension and in Dynamic Cultures.- Differentiation of Pluripotent Stems Cells in vitro: Embryoid Bodies.- Differentiation of Pluripotet Stems Cells in vivo: Teratoma Formation.- Immunostaining.- Karyotype Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Lines.- Method for the Derivation of Induced Pluripotent Stem Cells from Human Hair Follicle Keratinocytes.

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Methods for the Derivation of Human Embryonic Stem Cell Lines.- Morphology of Human Embryonic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders.- Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder-Layer-Free Conditions.- Morphology of Undifferentiated Human Embryonic and Induced Stem Cells Grown in Suspension and in Dynamic Cultures.- Differentiation of Pluripotent Stems Cells in vitro: Embryoid Bodies.- Differentiation of Pluripotet Stems Cells in vivo: Teratoma Formation.- Immunostaining.- Karyotype Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Lines.- Method for the Derivation of Induced Pluripotent Stem Cells from Human Hair Follicle Keratinocytes.

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Methods for the Derivation of Human Embryonic Stem Cell Lines.- Morphology of Human Embryonic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders.- Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder-Layer-Free Conditions.- Morphology of Undifferentiated Human Embryonic and Induced Stem Cells Grown in Suspension and in Dynamic Cultures.- Differentiation of Pluripotent Stems Cells in vitro: Embryoid Bodies.- Differentiation of Pluripotet Stems Cells in vivo: Teratoma Formation.- Immunostaining.- Karyotype Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell Lines.- Method for the Derivation of Induced Pluripotent Stem Cells from Human Hair Follicle Keratinocytes.

Key concepts: Embryoid body, Induced pluripotent stem cell, Embryonic stem cell, KOSR, Stem cell, Biology, Cell biology, Adult stem cell

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