Transgenesis techniques : principles and protocols

Part I: Transgenesis in Various Model Systems 1. Transgenesis in Drosophila melanogaster Leonie Ringrose 2. Transgenesis in Caenorhabditis elegans Matthias Rieckher, Nikos Kourtis, Angela Pasparaki, and Nektarios Tavernarakis 3. Transgenesis in Zebrafish with the Tol2 Transposon System Maximiliano L. Suster, Hiroshi Kikuta, Akihiro Urasaki, Kazuhide Asakawa, and Koichi Kawakami 4. Generation of Transgenic Frogs Jana Loeber, Fong Cheng Pan, and Tomas Pieler 5. Pronuclear DNA Injection for the Production of Transgenic Rats Jean Cozzi, Ignacio Anegon, Valerie Braun, Anne-Catherine Gross, Christel Merrouche, and Yacine Cherifi Part II: Transgenesis in the Mouse 6. Cell-Type Specific Transgenesis in the Mouse James Gulick and Jeffrey Robbins 7. Transgene Design and Delivery into the Mouse Genome: Keys to Success Lydia Teboul 8. Overexpression Transgenesis in Mouse: Pronuclear Injection Wendy J.K. Gardiner and Lydia Teboul 9. Gene Targeting Vectors J. Simon C. Arthur and Victoria A. McGuire 10. Gene Trap: Knockout on the Fast Lane Melanie Ullrich and Kai Schuh 11. Culture of Murine Embryonic Stem Cells Ivana Barbaric and T. Neil Dear 12. Targeting Embryonic Stem Cells Roland H. Friedel 13. Generation of Chimeras by Microinjection Anne Pluck and Christian Klasen 14. Generation of Chimeras by Morula Aggregation Anne Pluck and Christian Klasen 15. Surgical Techniques for the Generation of Mutant Mice Anne Pluck and Christian Klasen 16. Site-specific Recombinases for Manipulation of the Mouse Genome Marie-ChristineBirling, Francoise Gofflot, and Xavier Warot 17. Cre Transgenic Mouse Lines Xin Wang 18. Large-Scale Mouse Mutagenesis Elizabeth J. Cartwright 19. Dedicated Mouse Production and Husbandry Lucie Vizor and Sara Wells 20. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice: Part I - Conserving Mutant Strains Martin D. Fray 21. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice: Part II - Recovery and Distribution of Conserved Mutant Strains Martin D. Fray