Nuclear reprogramming : methods and protocols

Nuclear Transplantation in Xenopus John B. Gurdon Nuclear Transfer in Sheep William A. Ritchie Protocols for Nuclear Transfer in Mice Shaorong Gao Isolation of Stromal Stem Cells From Human Adipose Tissue Andrew C. Boquest, Aboulghassem Shahdadfar, Jan E. Brinchmann, and Philippe Collas Nuclear Reprogramming by Cell Fusion Robert H. Broyles, Austin C. Roth, Mairead Todd, and Visar Belegu Polyethylene Glycol-Mediated Cell Fusion Jian Yang and Ming Hong Shen Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells Masako Tada and Takashi Tada Quantification of Cell Fusion by Flow Cytometry Stephen Sullivan, Martin Waterfall, Ed J. Gallagher, Jim McWhir, and Steve Pells Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts Anne-Mari Hakelien, Kristine G. Gaustad, and Philippe Collas Transgenic Systems in Nuclear Reprogramming Megan Munsie, Peter Mountford, and Jennifer Nichols Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos Fatima Santos and Wendy Dean Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies Rong Wu, Anna V. Terry, and David M. Gilbert Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones Dean H. Betts, Steven Perrault, Lea Harrington, and W. Allan King Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells Cornelia Wiese, Gabriela Kania, Alexandra Rolletschek, Przemyslaw Blyszczuk, and Anna M. Wobus Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens Bruce A. Fenderson, Maria P. De Miguel, April D. Pyle, and Peter J. Donovan Analysis of the Nucleolar Compartment of the Nucleus as anIndicator of Nuclear Reprogramming After Nuclear Transfer Jacques-E. Flechon Methylation-Sensitive Polymerase Chain Reaction Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang Chromatin Immunoprecipitation Assay for Mammalian Tissues Fiona B. Turner, Wang L. Cheung, and Peter Cheung Histone Modifications and Transcription Factor Binding on Chromatin: ChIP-PCR Assays Jaejoon Won and Tae Kook Kim In Vivo Genomic Footprinting Using LM-PCR Methods Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease Pascal Lefevre and Constanze Bonifer Index