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2013•Kyokugen Kankyo Seibutsu GakkaishiOpen access

Gene expression and characterization of aerotaxis transducer HemAT from extremely halophilic archaeon Haloarcula japonica TR-1

T Tadikara, Tatsuya Matsubara, Yoshihisa Kubota, Taiichiro Kosaka, Tomomi Ozawa, Wataru Tsukimura, Rie Yatsunami, Toshiaki Fukui, Kaoru Nakasone, Tomonori Takashina, Satoshi Nakamura

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Abstract

An open reading frame encoding an aerotaxis transducer HemAT homolog (named HemAT-Hj) was found in the genome of extremely halophilic archaeon Haloarcula japonica TR-1. HemAT-Hj would be a cytoplasmic protein consisting of a sensor domain, a linker region and a methyl-accepting signaling domain. The hemAT-Hj gene was successfully expressed in Escherichia coli. Recombinant HemAT-Hj was purified and characterized. Absorption spectra showed that E. coli-produced HemAT-Hj contained a b-type heme molecule.

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An open reading frame encoding an aerotaxis transducer HemAT homolog (named HemAT-Hj) was found in the genome of extremely halophilic archaeon Haloarcula japonica TR-1. HemAT-Hj would be a cytoplasmic protein consisting of a sensor domain, a linker region and a methyl-accepting signaling domain. The hemAT-Hj gene was successfully expressed in Escherichia coli. Recombinant HemAT-Hj was purified and characterized. Absorption spectra showed that E. coli-produced HemAT-Hj contained a b-type heme molecule.

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Available abstract

An open reading frame encoding an aerotaxis transducer HemAT homolog (named HemAT-Hj) was found in the genome of extremely halophilic archaeon Haloarcula japonica TR-1. HemAT-Hj would be a cytoplasmic protein consisting of a sensor domain, a linker region and a methyl-accepting signaling domain. The hemAT-Hj gene was successfully expressed in Escherichia coli. Recombinant HemAT-Hj was purified and characterized. Absorption spectra showed that E. coli-produced HemAT-Hj contained a b-type heme molecule.

Key concepts: Halophile, Gene, Biology, Japonica, Gene expression, Genetics, Bacteria, Botany

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Gene expression and characterization of aerotaxis transducer HemAT from extremely halophilic archaeon Haloarcula japonica TR-1 — Research Paper | ScholarLens