1994Unpublished venueRequires access

In situ hybridization in neurobiology : advances in methodology

James Eberwine, Karen L. Valentino, Jack D. Barchas

Open publisher page 14 citations

Abstract

1. Methodological considerations in the utilization of In situ hybridization 2. Combining non-isotopic In situ hybridization histochemistry with steroid autoradiography 3. Development of techniques to combine isotopic and non-isotopic In situ hybridization and immunocytochemistry for phenotypic characterization of individual neurons 4. Enzyme histochemical detection of neuronal mRNA 5. Characterization of the receptor complement of individual neurons using dual-label In situ hybridization histochemistry 6. Assessment of peptide secretion and gene expression within individual cells 7. Quantitative micro-In situ hybridation 8. Quantification of In situ hybridization histochemistry 9. Analysis of transcription levels using intervening sequence In situ hybridization 10. Analysis of alternatively-transcribed messages in brain by In situ hybridization using biotinylated oligonucleotide probes 11. In situ polymerase chain reaction: A powerful new methodology 12. In situ transcription: Methodology and multi-faceted applications

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What this paper is about

1. Methodological considerations in the utilization of In situ hybridization 2. Combining non-isotopic In situ hybridization histochemistry with steroid autoradiography 3. Development of techniques to combine isotopic and non-isotopic In situ hybridization and immunocytochemistry for phenotypic characterization of individual neurons 4. Enzyme histochemical detection of neuronal mRNA 5. Characterization of the receptor complement of individual neurons using dual-label In situ hybridization histochemistry 6. Assessment of peptide secretion and gene expression within individual cells 7. Quantitative micro-In situ hybridation 8. Quantification of In situ hybridization histochemistry 9. Analysis of transcription levels using intervening sequence In situ hybridization 10. Analysis of alternatively-transcribed messages in brain by In situ hybridization using biotinylated oligonucleotide probes 11. In situ polymerase chain reaction: A powerful new methodology 12. In situ transcription: Methodology and multi-faceted applications

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Available abstract

1. Methodological considerations in the utilization of In situ hybridization 2. Combining non-isotopic In situ hybridization histochemistry with steroid autoradiography 3. Development of techniques to combine isotopic and non-isotopic In situ hybridization and immunocytochemistry for phenotypic characterization of individual neurons 4. Enzyme histochemical detection of neuronal mRNA 5. Characterization of the receptor complement of individual neurons using dual-label In situ hybridization histochemistry 6. Assessment of peptide secretion and gene expression within individual cells 7. Quantitative micro-In situ hybridation 8. Quantification of In situ hybridization histochemistry 9. Analysis of transcription levels using intervening sequence In situ hybridization 10. Analysis of alternatively-transcribed messages in brain by In situ hybridization using biotinylated oligonucleotide probes 11. In situ polymerase chain reaction: A powerful new methodology 12. In situ transcription: Methodology and multi-faceted applications

Key concepts: In situ hybridization, In situ, Biology, Immunocytochemistry, Molecular biology, Biotinylation, Oligomer restriction, Riboprobe

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