In situ hybridization in neurobiology : advances in methodology
James Eberwine, Karen L. Valentino, Jack D. Barchas
Abstract
James Eberwine, Karen L. Valentino, Jack D. Barchas
Abstract
1. Methodological considerations in the utilization of In situ hybridization 2. Combining non-isotopic In situ hybridization histochemistry with steroid autoradiography 3. Development of techniques to combine isotopic and non-isotopic In situ hybridization and immunocytochemistry for phenotypic characterization of individual neurons 4. Enzyme histochemical detection of neuronal mRNA 5. Characterization of the receptor complement of individual neurons using dual-label In situ hybridization histochemistry 6. Assessment of peptide secretion and gene expression within individual cells 7. Quantitative micro-In situ hybridation 8. Quantification of In situ hybridization histochemistry 9. Analysis of transcription levels using intervening sequence In situ hybridization 10. Analysis of alternatively-transcribed messages in brain by In situ hybridization using biotinylated oligonucleotide probes 11. In situ polymerase chain reaction: A powerful new methodology 12. In situ transcription: Methodology and multi-faceted applications
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1. Methodological considerations in the utilization of In situ hybridization 2. Combining non-isotopic In situ hybridization histochemistry with steroid autoradiography 3. Development of techniques to combine isotopic and non-isotopic In situ hybridization and immunocytochemistry for phenotypic characterization of individual neurons 4. Enzyme histochemical detection of neuronal mRNA 5. Characterization of the receptor complement of individual neurons using dual-label In situ hybridization histochemistry 6. Assessment of peptide secretion and gene expression within individual cells 7. Quantitative micro-In situ hybridation 8. Quantification of In situ hybridization histochemistry 9. Analysis of transcription levels using intervening sequence In situ hybridization 10. Analysis of alternatively-transcribed messages in brain by In situ hybridization using biotinylated oligonucleotide probes 11. In situ polymerase chain reaction: A powerful new methodology 12. In situ transcription: Methodology and multi-faceted applications
Key concepts: In situ hybridization, In situ, Biology, Immunocytochemistry, Molecular biology, Biotinylation, Oligomer restriction, Riboprobe