A review on protocols for in vitro regeneration of plants

In the present review, an attempt has been made to set up protocols for in vitro regeneration of plant from young leaves, seedlings as explant source. For in vitro callus inductions auxins such as IAA, NAA and 2, 4-D in combination with cytokines like BAP and KN areused. Awell-developed greenish plant is obtained on MS medium when supplemented with BAP and NAA. The maximum number of shoot bud regeneration (14.0 shoots/explant) is observed on MS medium containing 5.0mg/l BAP, ofthe two cytokines used, BAP is found to be best for shoot bud regeneration. For root induction, elongated shoots were transferred to MS medium supplemented with various concentrations of (0.5–2.0 mg/l) IAA, IBA and NAA and in combination with 0.5 mg/l BAP and KIN on half strength MS medium. Among the different concentrations of auxin tested NAA was induced maximum percent of rooting (100%). The maximum number of roots (9.0 roots/shoot) was observed on MS medium supplemented with 0.5 mg/l NAA. Rooted plantlets were transferred into soil and sand in the ratio (2:1) and subsequently, survival plants were acclimatized in the field.