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2005•Acta Scientiarum Naturalium Universitatis SunyatseniRequires access

RAPD Analysis for Genetic Variation within the Endangered Quillwort Isoetes hypsophila （Isoetaceae）

CHENJin-ming, WANGJing-yuan, LIU-Xing, GituruWahitiRobert, WangQing-feng

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Abstract

The genetic variation in the critically endangered species Isoetes hypsophila was investigated using Random Amplified Polymorphism DNA (RAPD) markers. Thirteen primers were screened from sixty primers, and a total of 104DNA fragments were scored, of which, 52 were polymorphic loci. Low-level genetic diversity within populations with PPB values ranging from 7.69% to 25.96% was found. An Anal ysis of Molecular Variance (AMOVA) indicated that the most of variance (78. 30%) occurred between Yunnan and Sichuan. The variances among populations within regions and within populations were only 3. 89% and 17. 82%, respectively.

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What this paper is about

The genetic variation in the critically endangered species Isoetes hypsophila was investigated using Random Amplified Polymorphism DNA (RAPD) markers. Thirteen primers were screened from sixty primers, and a total of 104DNA fragments were scored, of which, 52 were polymorphic loci. Low-level genetic diversity within populations with PPB values ranging from 7.69% to 25.96% was found. An Anal ysis of Molecular Variance (AMOVA) indicated that the most of variance (78. 30%) occurred between Yunnan and Sichuan. The variances among populations within regions and within populations were only 3. 89% and 17. 82%, respectively.

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Available abstract

The genetic variation in the critically endangered species Isoetes hypsophila was investigated using Random Amplified Polymorphism DNA (RAPD) markers. Thirteen primers were screened from sixty primers, and a total of 104DNA fragments were scored, of which, 52 were polymorphic loci. Low-level genetic diversity within populations with PPB values ranging from 7.69% to 25.96% was found. An Anal ysis of Molecular Variance (AMOVA) indicated that the most of variance (78. 30%) occurred between Yunnan and Sichuan. The variances among populations within regions and within populations were only 3. 89% and 17. 82%, respectively.

Key concepts: RAPD, Analysis of molecular variance, Endangered species, Biology, Genetic diversity, Genetic variation, Botany, Zoology

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RAPD Analysis for Genetic Variation within the Endangered Quillwort Isoetes hypsophila （Isoetaceae） — Research Paper | ScholarLens