Studies on gametophyte development and spore propagation of Aleuritopteris argentea.

The effects of different cultivation conditions on spore germination,gametophyte development and sporophyte growth of Aleuritopteris argentea were studied. The results showed that the spore germination is Vittaria-type while the gametophyte developing is Ceratopteris-type. No significant differences were observed in germination rate and time among spores stored at 4 ℃ with periods of 0,2,4,6 and 10 months respectively. The studies on four general solid substrates showed that the growth and development of gametophytes failed either in soil or in peat alone,while the maximum rates of spore germination and sporophyte formation were obtained in the mixed substrate(the peat and the sand mixed in volume ratio of 1∶1) ,up to 65% and 83.2% respectively. Investigation about the sterile cultivation condition found that,of all the mediums with different inorganic salt concentration,MS basal medium is the best for spore germination(reached 53.3%) and gametophyte expansion. When the concentration decreased to 1/8MS,the spores failed to geminate.The results of different sucrose concentrations from 0% to 4% in MS medium on gametophyte growth showed that,4% was optimum in improving gametophyte expansion,increasing embryo inducing and shortening the period from gametophyte to sporophyte.