2011•Methods in molecular biologyRequires access

Profiling Cellular Myristoylation and Palmitoylation Using ω-Alkynyl Fatty Acids

Rami N. Hannoush

Abstract

Methods to detect and characterize cellular protein myristoylation and palmitoylation are invaluable in cell biology, immunology, and virology. Recently, we developed ω-alkynyl fatty acid probes for monitoring myristoylation and palmitoylation of cellular proteins. This article describes a biochemical procedure for metabolic labeling of cells with ω-alkynyl fatty acids and click chemistry.

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Key concepts: Palmitoylation, Myristoylation, Biochemistry, Click chemistry, Chemistry, Fatty acid, Cell biology, Biology