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2009•Zhongguo linchuang baojian zazhiRequires access

Isolation and biological characteristics detection of CD4- CD8- T cells in human peripheral blood.

Jiong Chen, Min Du, Wenbo Li, Hou-kuo Tang, Bing Yang, Zhao ZhiMin, Qing Li

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Abstract

Objective To isolate,culture and detect the biological activity of CD4-CD8-T cells.Methods Immuno-magnetic beads and flow cytometry methods were used to isolate and analyze CD4-CD8-T cells.ELISA method was used to detect the levels of IFN-γ and IL-4 secreted by CD4-CD8-T cells.Results The purity of CD4-CD8-T cells was 82.77%,and the content of IFN-γ secreted by CD4-CD8-T cells was 159.28±38.16 pg/ml,but IL-4 secreted by CD4-CD8-T cells was not detected.Conclusion CD4-CD8-T cells could secrete IFN-γ,not IL-4.

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What this paper is about

Objective To isolate,culture and detect the biological activity of CD4-CD8-T cells.Methods Immuno-magnetic beads and flow cytometry methods were used to isolate and analyze CD4-CD8-T cells.ELISA method was used to detect the levels of IFN-γ and IL-4 secreted by CD4-CD8-T cells.Results The purity of CD4-CD8-T cells was 82.77%,and the content of IFN-γ secreted by CD4-CD8-T cells was 159.28±38.16 pg/ml,but IL-4 secreted by CD4-CD8-T cells was not detected.Conclusion CD4-CD8-T cells could secrete IFN-γ,not IL-4.

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Available abstract

Objective To isolate,culture and detect the biological activity of CD4-CD8-T cells.Methods Immuno-magnetic beads and flow cytometry methods were used to isolate and analyze CD4-CD8-T cells.ELISA method was used to detect the levels of IFN-γ and IL-4 secreted by CD4-CD8-T cells.Results The purity of CD4-CD8-T cells was 82.77%,and the content of IFN-γ secreted by CD4-CD8-T cells was 159.28±38.16 pg/ml,but IL-4 secreted by CD4-CD8-T cells was not detected.Conclusion CD4-CD8-T cells could secrete IFN-γ,not IL-4.

Key concepts: CD8, Flow cytometry, Molecular biology, Secretion, Medicine, T lymphocyte, Cytotoxic T cell, Immunology

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Isolation and biological characteristics detection of CD4- CD8- T cells in human peripheral blood. — Research Paper | ScholarLens