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2010•Unpublished venueRequires access

Expression of Recombinant Human Stem Cell Factor (hSCF) Protein using Bombyx mori Protein Disulfide Isomerase (bPDI)

Sung Wan Kim, Eun Young Yun, Seong‐Ryul Kim, Seung Won Park, Seok Woo Kang, Kwang Gill Lee, O‐Yu Kwon, Tae Won Goo

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Abstract

Protein disulfide isomerase (PDI) catalyzes the oxida-tion of disulfides and the isomerizatiob of incorrect disulfides in new polypeptides during folding in the oxidizing environment of the endoplasmic reticulum (ER). To increase recombinant protein hSCF (human stem cell factor) production, we have developed expression system using the Bombyx mori PDI (bPDI) as a fusion partner. bPDI gene fusion was found to improve the production of recombinant hSCFs. Thus, we conclude that bPDI gene fusion will be very useful for the large-scale production of biologically active recombinant proteins.

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What this paper is about

Protein disulfide isomerase (PDI) catalyzes the oxida-tion of disulfides and the isomerizatiob of incorrect disulfides in new polypeptides during folding in the oxidizing environment of the endoplasmic reticulum (ER). To increase recombinant protein hSCF (human stem cell factor) production, we have developed expression system using the Bombyx mori PDI (bPDI) as a fusion partner. bPDI gene fusion was found to improve the production of recombinant hSCFs. Thus, we conclude that bPDI gene fusion will be very useful for the large-scale production of biologically active recombinant proteins.

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Available abstract

Protein disulfide isomerase (PDI) catalyzes the oxida-tion of disulfides and the isomerizatiob of incorrect disulfides in new polypeptides during folding in the oxidizing environment of the endoplasmic reticulum (ER). To increase recombinant protein hSCF (human stem cell factor) production, we have developed expression system using the Bombyx mori PDI (bPDI) as a fusion partner. bPDI gene fusion was found to improve the production of recombinant hSCFs. Thus, we conclude that bPDI gene fusion will be very useful for the large-scale production of biologically active recombinant proteins.

Key concepts: Protein disulfide-isomerase, Biology, Bombyx mori, Fusion protein, Recombinant DNA, Endoplasmic reticulum, Gene, Isomerase

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