Peptide research protocols : endothelin

Part I. Endothelin Peptide Protocols Immunocytochemical Localization of Endothelin Peptides, Precursors, and Endothelin-Converting Enzymes Rhoda E. Kuc Analysis of Endothelins by Enzyme-Linked Immunosorbent Assay and Radioimmunoassay Anthony P. Davenport and Rhoda E. Kuc Separation of Endothelin Isoforms by High Performance Liquid Chromatography and Detection by Radioimmunoassay Michael J. Ashby Part II. Endothelin Receptor Protocols Radioligand Binding Assays and Quantitative Autoradiography of Endothelin Receptors Anthony P. Davenport and Rhoda E. Kuc Using Receptor Antagonists in Binding Studies to Characterize a Mammalian Endothelin Receptor J. Ruth Wu-Wong Quantification of Endothelin Receptor mRNA by Competitive RT-PCR Paula J. W. Smith and Juan Carlos Monge Part III. Endothelin-Converting Enzyme Protocols Detection and Assessment of Endothelin-Converting Enzyme Activity Carolyn D. Jackson and Anthony J. Turner Quantitative Measurement of mRNA Levels by RT-PCR: Studies of ECE-1 Isoforms Delphine M. Lees, Noorafza Q. Khan, Stewart Barker, and Roger Corder Evaluation of Endothelin-Converting Enzyme Inhibitors Using Cultured Cells, Roger Corder Endothelin-Converting Enzyme Activity in Vascular Smooth Muscle Preparations In Vitro Janet J. Maguire Part IV. In Vitro Functional Assays Characterization of a New Endothelin Receptor Ligand by In Vitro Assays J. Ruth Wu-Wong Use of In Vitro Organ Cultures of Human Saphenous Vein as a Model for Intimal Proliferation Karen E. Porter Part V. In Vivo Functional Assays Investigation of the Endothelin System in Experimental Heart Failure Gillian A. Gray and Lorcan Sherry Local Forearm Vasoconstriction to Endothelin-1 Measured by Venous Occlusion Plethysmography In Vivo Fiona E. Strachan and David J. Webb Index