Peptide antigens : a practical approach

Peptide antigens and anti-peptide antibodies are widely used in biochemistry and molecular biology for the measurement, location, and purification of specific oligopeptides. More recently the application of these reagents has expanded, for example in the identification and mapping of the binding sites of antibodies and T-cell receptors and in the identification and characterization of proteins which are known only by their primary structure. This volume provides practical guidance to the major techniques used in the exploitation of peptide antigens and anti-peptide antibodies. The chapters give detailed protocols for the prediction of epitopes, peptides synthesis, the preparation of peptide immunogens, immunoaffinity chromatography, immunoassays, and the mapping of epitopes using both synthetic peptides and phage display systems