Study on the value of exogenous bFGF in the treatment of brain injury

Objective:To investigate the relation between necrosis and apoptosis in the hippocampus of exogenous bFGF on this process.Methos:With Marmarou's method we produced a severe diffuse brain injury and studied the changes in the hippocampus by adapting a modified TdT-mediated dUTP-biotin nick end labeling (TUNEL) method.At the same time we observed the effect of exogenous bFGF on neuronal necrosis and apoptosis.Results:We found that together with cell necrosis there was an increase in the number of apoptotic neurons in the hippocampus CA2-3 sectors as early as 4h after injury,with numbers reaching a maximum at 7d.Exogenous bFGF resulted in a definite reduction in the amount of necrosis and apoptosis.Conclusions:Neuronal necrosis and apoptosis occur in combination after brain injury and that one of the causes may be the insufficience expression of the bFGF gene in the hippocampus after severe injury.Exogenous bFGF and similar substance may prove clinically useful after brain injury by reducing cell necrosis and apoptosis.