Effect of different enzymes and optimization of cultural conditions on xylose production using response surface methodology (RSM)

Xylose is found in the embryos of most edible plants. Xylan, the hemicellulose used as raw material in xylose production. Xylanase, the enzyme that is used to hydrolyzed the structure of xylan to produce xylose. This study aims to improve the production of xylose from Palm Oil Empty Fruit Bunch (POEFB) using enzymatic hydrolysis process. The effect of cultural conditions such as enzyme concentration and incubation time are used to study the production of xylose. Three different xylanases enzyme are used to produce xylose namely Multifect Xylanase, Multifect CX 12L and Optimase CX 72L. Multifect CX 12L was the best enzyme that yielded the most xylose.The optimization of the production of xylose is carried out by using response surface methodology (RSM) based on the central composite design (CCD). The optimal set cultural conditions for enzyme concentration are 50 U/g and 35.5 hours of incubation. Xylose production of 23.15 mg/ml was achieved by using these optimal conditions.