2009•Chinese Veterinary ScienceRequires access
Analyses of variability of eight gene fragments of influenza A (H1N1) virus A/New York/1669/2009 (H1N1) and its pandemicity.
Yao Bi, Hailong Chen, Kun Liu, Zhang Lezhi, Zhao Yujun, Ma XingYuan
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Abstract
In order to explore the internus reasons of the recent pandemic influenza and the variability of eight gene fragments of influenza A(H1N1) virus,a typical influenza A(H1N1) virus A/NewYork/1669/2009(H1N1) broke out recently was analyzed and compared on its eight gene fragments of PA,NP,M,PB1,PB2,NA,HA,and NS by BLAST and CLUSTAL X bioinformatics softwares.The results showed that PB1 and NP genes derived from human influenza virus,and HA,NA,NS,and M genes derived from swine influenza virus,while PB2 and PA genes derived from avian influenza virus.A new glycosylation site-NTT-was found in position site 249 of HA gene,and another new glycosylation site-NFSI-was found in position site 386 of NA gene,too.Meanwhile,the changes of 3 amino acids on HA gene led to the changes of 6 potential antigenic determinants,and 5 of 12 potential antigenic determinants of NA gene changed.The recombination and high-frequency variability of these antigenic determinants might be the primary cause of the pandemic influenza outbreak.