2004•World Chinese Journal of DigestologyOpen access
Gene expression profiles in liver cirrhosis and normal liver tissues
Lianxin Liu, Zhi-Hong Chen, Linfeng Wu, Hong-Wei Li, Zhi-Hua Liu, Hongchi Jiang, Xiuqin Wang, Min Wu
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Abstract
AIM: To describe liver specific gene expression profiles and to identify genes with differential expression between liver cirrhotic tissues and normal liver tissues. METHODS: The cDNA probes which were labeled with α32 P dATP were synthesized from total RNAs of liver cirrhosis and normal liver tissues and hybridized to two identical Atlas human cDNA expression arrays membranes containing 588 known genes respectively. RESULTS: Autoradiographic results were analyzed by specific AtlasImage TM (version1.01a) software. Among the 588 genes analyzed, 17 genes were found up-regulated in cirrhosis, including integrin beta 7 and collagen type XVIII, and 98 genes were down-regulated in cirrhosis, including TFDP2, BAK and ABL. Expression of the genes was associated with the regulation of cell proliferation, apoptosis, differentiation, cell-cell interaction, invasion regulators and cytokines altered. CONCLUSION: The results obtained from Atlas microarray provide a comprehensive liver cirrhosis specific expression profile. These results may be helpful for identification of target genes for diagnosis and designing rational therapeutic