Flavonoids from the bran of Avena sativa

Abstract Aim To investigate the chemical constituents from the bran of Avena sativa. Methods Compounds were isolated and purified by various column chromatographic techniques using macroporous resin, silica gel, Sephadex LH-20, ODS, and preparative reversed-phase HPLC. Their structures were elucidated by physicochemical properties and spectroscopic data. Results Fifteen chemical constituents were isolated and identified as kaempferol 3- O -(2″, 3″-di-E-p-coumaroyl)- a -L-rhamnopyranoside ( 1 ), kaempferol 3- O -(3″-E-p-coumaroyl)- a -L-rhamnopyranoside ( 2 ), kaempferol 3- O -(2″-O-E-p-coumaroyl)-β-D-glucopyranoside ( 3 ), kaempferol 3-O-β-D-glucopyranoside ( 4 ), kaempferol 7- O-a -L-rhamnopyranoside ( 5 ), linarin ( 6 ), tilianin ( 7 ), myricitrin ( 8 ), quercitrin ( 9 ), kaempferol 3- O -rutinoside ( 10 ), rutin ( 11 ), tricin 7- O-β -D-glucopyranoside ( 12 ), tricin ( 13 ), kaempferol ( 14 ), and luteolin ( 15 ). Conclusion Compounds 1-9 were isolated from Avena sativa Linn. for the first time.