2023•FigshareOpen access
Gene models for Drosophila ananassae, Drosophila bipectinata, Drosophila kikkawai, and Drosophila takahashii
Wilson Leung, Nicole S. Torosin, Weihuan Cao, Laura K Reed, Cindy Arrigo, Sarah C. R. Elgin, Christopher E. Ellison
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Abstract
These files are gene annotations for the genome assemblies described in the forthcoming publication Long-read genome assemblies for the study of chromosome expansion: Drosophila kikkawai, Drosophila takahashii, Drosophila bipectinata, and Drosophila ananassae. RefSeq gene models from D. kikkawai, D. takahashii, D. bipectinata, and D. ananassae were aligned against the corresponding Hi-C genome assembly using BLAT with the following parameters q=rna -fine -minScore=20 -stepSize=5. The transcript alignments were analyzed by pslReps with the parameters -minCover=0.15 -minAli=0.98 -nearTop=0.001, and then filtered by pslCDnaFIlter with the parameters -minId=0.95 -minCover=0.15 -localNearBest=0.001 -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap.