PLACKETT-BURMAN DESIGN FOR SCREENING MEDIA COMPONENTS FOR ALKALINE PROTEASE PRODUCTION FROM BACILLUS LICHENIFORMIS THROUGH SUBMERGED FERMENTATION
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Abstract
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Abstract
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Bacillus licheniformis SCJ4, a marine bacteria isolated from highly polluted area of Lucknow, showed a high potency for contemporaneous production of alkaline protease enzyme.In the current study, Plackett-Burman factorial design was applied to evaluate culture conditions affecting the production of enzyme.Analysis of Plackett-Burman design results revealed that, the most significant variables affecting alkaline protease production were glucose, K2HPO4, MgSO4 and CaCl2 with more than 6 folds increase in enzyme activity than basal medium (1185 Uml-1min-1 after 48h).
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Bacillus licheniformis SCJ4, a marine bacteria isolated from highly polluted area of Lucknow, showed a high potency for contemporaneous production of alkaline protease enzyme.In the current study, Plackett-Burman factorial design was applied to evaluate culture conditions affecting the production of enzyme.Analysis of Plackett-Burman design results revealed that, the most significant variables affecting alkaline protease production were glucose, K2HPO4, MgSO4 and CaCl2 with more than 6 folds increase in enzyme activity than basal medium (1185 Uml-1min-1 after 48h).
Key concepts: Bacillus licheniformis, Plackett–Burman design, Alkaline protease, Fermentation, Food science, Protease, Chemistry, Biotechnology