A Novel Method for Extraction of High Purity and High Production Phytophthora sojae Oospores

Abstract Background: Phytophthora sojae, a soilborne oomycete pathogen, has been a yield limiting factor for more than 60 years on soybean. The resurgence of P. sojae（Phytophthora sojae）is primarily ascribed to the durable oospores found in soil and remnants of the disease. P. sojae is capable of infesting at any growth periods of the soybean, and the succeed infestation of P. sojae is predominantly attributed to long-lived oospores present in soil. Comprehending the molecular mechanisms that drive oospore formation and their significance in infestation is vital for effectively managing the varied diseases it causes. However, the existing challenges in isolating and extracting significant quantities of oospores pose limitations in investigating the sexual reproductive stages of P. sojae. Results: This study focused on optimizing and refining the culture conditions and extraction process of P. sojae, leading to the establishment of an efficient and dependable method for extracting this organism. Optimized novel approachyielded greater quantitiesof high-purity P. sojae than traditional methods. Fortunately, the viability of newly approached oospores did not differ significantly from that of the traditional one, as much the same survival and germination rates of new oospores were detected in deficiency obstructs project implementation (MTT) and plate coating assays. Moreover, in potting experiments, no drastically difference in the pathogenicity of oospores was conducted between the novel and traditional method. Conclusion: Consequently, the proposed method for extracting P. sojae oospores efficiently yields a substantial quantity of highly pure, viable, and pathogenic oospores. The enhancements in oospore extraction techniqueswill promote the research on the sexual reproductive mechanisms of P. sojaeand lead to the creation of innovative and effective approaches for managing oomycete diseases.