Evaluation of Extenders for Cryopreservation of Cirrhinus mrigala Milt

This study aimed to evaluate the performance of different extenders for the cryopreservation of Cirrhinus mrigala milt. The extenders under investigation included Kurokura-2 + DMSO (KD), Alsever`s solution + DMSO (AD), Kurokura-2 + Methanol (KM), and Alsever`s solution + Methanol (AM). Sperm quality assessments encompassed critical parameters such as sperm motility, motility duration, viability, and DNA integrity. The initial analysis of fresh milt revealed motility rate of 88.33±1.66%, pH of 7.52±0.76, a volume of 7.83±0.44 mL, a sperm count of 3.55±0.40 x 1010/mL. Upon thawing, it became evident that the KD extender yielded significantly higher post-thaw sperm motility (%) at 68±1.67, compared to the KM (40.00±2.89), AD (38±1.67), and AM (41.67±4.40). Additionally, sperm motility duration (seconds) exhibited a notable increase in the KD extender (78.67±10.413) in comparison to the KM (52.00±5.291), AD (46.66±1.66), and AM (41.67±1.667) extenders. The KD extender also contributed to higher sperm viability (%) at 66.00±1.00, whereas KM and AM exhibited similar values at 59.00±3.00 and 55.33±6.38, respectively. Furthermore, DNA integrity (%) remained consistently high for KD, KM, and AM (98.67±0.66, 98.00±1.52%, and 97.33±1.20%), while a lower percentage was observed for AD (88.00±1.15). In conclusion, the modified Kurokura extender, supplemented with 10% DMSO, showcased a pronounced positive impact on the cryopreservation of Cirrhinus mrigala milt.