Comparative Evaluation of Titanium-platelet-rich Fibrin and Leukocyte-platelet-rich Fibrin – A Scanning Electron Microscopic Study

A BSTRACT Background: Titanium-prepared platelet-rich fibrin (T-PRF), a new autologous platelet-rich fibrin (PRF) product, is based on the hypothesis that titanium tubes may be more effective in activating platelets when compared with Choukroun’s leukocyte and PRF (L-PRF) method. In this study, we aimed to define the structural characteristics under scanning electron microscopy of T-PRF and compare it with L-PRF. Materials and Methods: Blood samples were collected from 10 healthy volunteers. The blood samples were drawn using a 10 ml syringe. A volume of 9 ml was transferred to a dry glass tube, and 9 ml was transferred to a titanium tube and centrifuged. Half of each clot (i.e. after centrifugation, the T-PRF and L-PRF clots were collected) samples were processed with a scanning electron microscope analysis. Results: The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Conclusion: Histomorphometry analysis showed that the T-PRF fibrin network covers a larger area than the L-PRF fibrin network, and also, fibrin seemed thicker in the T-PRF samples. The platelet activation by titanium seems to offer some high characteristics to T-PRF. CLINICAL RELEVANCE TO INTERDISCIPLINARY DENTISTRY Titanium-prepared platelet-rich fibrin (T-PRF), A new autologous platelet fibrin rich product can be used in regeneration of pulpal and periodontal tissues.