Cell-Free Synthetic Biology

Innovations in sustainable biomanufacturing are made possible by cell-free synthetic biology advances. Cell-free workflows frequently combine purified enzymes with cell extracts for cell-free protein synthesis processes to create and analyze biosynthetic pathways and synthesize therapeutics. The development of cell-free platforms, high-yielding, cell-free gene expression systems, and multiplexed strategies for quickly evaluating biological design have all been made possible by recent technological advances in cell-free platforms, such as microfluidics, machine learning, CRISPR-mediated genome editing, etc. Developments in cell-free platforms satisfy many of the requirements of preparatory biochemical research, clinical purposes, diagnostic devices, industrial application, biosensors, etc. that offer exciting opportunities to transform synthetic biology and biotechnology. Therefore, this chapter aims to elaborate on the rapidly advancing field of cell-free platforms.