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2023•Unpublished venueOpen access

Quantifying Acetylcholinesterase activity in striatal brain tissue with DTNBassay v1

Stefania Vietti‐Michelina

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Abstract

This protocol describes the steps to measure acetylcholinesterase (AChE) activity in mouse striatal brain tissue, including how to store tissue samples, extract AChE and obtain Michaelis-Menten like plots to measure the rate of AChE activity. This protocol can be used to quantify AChE activity across different brain regions or genotypes. Reagents and materials from Abcam kit ab138871 are used. This kit uses DTNB to measure the thiocholine (TCh) resulting from the hydrolysis of the substrate acetylthiocholine (ATCh) by AChE.

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What this paper is about

This protocol describes the steps to measure acetylcholinesterase (AChE) activity in mouse striatal brain tissue, including how to store tissue samples, extract AChE and obtain Michaelis-Menten like plots to measure the rate of AChE activity. This protocol can be used to quantify AChE activity across different brain regions or genotypes. Reagents and materials from Abcam kit ab138871 are used. This kit uses DTNB to measure the thiocholine (TCh) resulting from the hydrolysis of the substrate acetylthiocholine (ATCh) by AChE.

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Available abstract

This protocol describes the steps to measure acetylcholinesterase (AChE) activity in mouse striatal brain tissue, including how to store tissue samples, extract AChE and obtain Michaelis-Menten like plots to measure the rate of AChE activity. This protocol can be used to quantify AChE activity across different brain regions or genotypes. Reagents and materials from Abcam kit ab138871 are used. This kit uses DTNB to measure the thiocholine (TCh) resulting from the hydrolysis of the substrate acetylthiocholine (ATCh) by AChE.

Key concepts: Acetylthiocholine, Acetylcholinesterase, Aché, DTNB, Brain tissue, Chemistry, Biochemistry, Enzyme

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