2023•Proceedings on CD-ROM - International Society for Magnetic Resonance in Medicine. Scientific Meeting and Exhibition/Proceedings of the International Society for Magnetic Resonance in Medicine, Scientific Meeting and ExhibitionRequires access
Hepatic and renal ketogenesis of hyperpolarized (1-13C)butyrate: functional evidence of metabolic zonation
Hikari A. I. Yoshihara, Rolf Gruetter, Verena Hoerr
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Abstract
Ketones bodies are an important metabolic fuel, and butyrate is a ketogenic short-chain fatty acid. We report the metabolism of hyperpolarized (1-13C)butyrate in the rat kidney and liver. The main metabolites in the kidney include [1-13C]acetoacetate, [1-13C]butyrylcarnitine and [5-13C]glutamate, and [1-13C]acetylcarnitine, 3-hydroxy[1-13C]butyrate, [5-13C]citrate and [3-13C]acetoacetate are also detectable. Fasting results in significantly lower butyrylcarnitine/[1-13C]acetoacetate and glutamate/[1-13C]acetoacetate ratios. Ketogenesis is observed in the liver and the [1-13C]butyrylcarnitine + [1-13C]acetoacetate signal normalized to butyrate is higher with fasting. The unexpectedly low 3-hydroxy[1-13C]butyrate signals are functional evidence of metabolic zonation, with acetoacetate production and reduction occurring in different cells.