Gibberellin of GA-4 from Bitter Gourd (Momordica charantia)

Gibberellin is one of phytohormone which has function to stimulate plant growth, especially for accelerating the growth of shoot and fruiting of plants.Gibberellin (GAs) are very important group of phytohormones involved in seed germination, vegetative growth, flowering and fruit development, being only four of the 136 known have more bio-active activity than other, they are GS-1, GA-3, GA-4 and GA-7.The objective of study is to isolate and identify gibberellin of GA-4 from bitter gourd (Momordica.Charantia), from Majalengka of West Java-Indonesia Plantation.Isolation and purification of GA-4 were carried out by thin-layer chromatography, adsorption column chromatography on Silica Gel G-60.while characterization of GA-4 were carried out by Reversed Phase High Performance Liquid Chromatography (RP-HPLC), Fourier Transform Infrared (FT-IR), and Mass Spectroscopy (MS).The results of RP-HPLC and FTIR of gibberelline of GA-4 isolates showed a retention time and wave number which was relatively similar to the GA-4 standard.From the result of MS analysis, the mass spectrum shows the peak of the molecular ion [M+H] + at m/z 355.1501 as the measured mass, and the calculated mass is 355.1521 with the molecular formula of C19H24O5Na, and the degree of saturation or DBE is 7.The measured molecular mass and the number of DBEs correspond to the molecular mass of GA-4.From the RP-HPLC found that GA-4 content in M.charantia was 6.06 mg/g dry weight.