Evaluation of Antimicrobial Activity of Citrus Leaves against Gram Positive and Gram Negative Bacterial Strains

In present study four bacterial strains, including Escherichia coli (Gram negative), Pseudomonas aeruginosa (Gram negative), Staphylococcus aureus (Gram positive), and Bacillus subtilis, were tested for antimicrobial activity of citrus leaf extracts in water, benzene, and methanol (Gram positive). According to the findings, Staphylococcus aureus (Gram positive) exhibited a 19 mm zone of inhibition in methanol extracts, followed by Bacillus subtilis (Gram positive) (16 mm). Staphylococcus aureus (Gram positive) showed zone of inhibition in benzene extracts was 17 mm, followed by Bacillus subtilis (Gram positive) (16 mm), and a similar pattern was seen in water extracts. Against Pseudomonas aeruginosa (a Gram negative bacteria), the zone of inhibition for gram negative bacteria was 12 mm in methanol extract, and a similar pattern was seen in benzene and water extracts.