Development and Validation by UV Spectrophotometric Method for Simultaneous Estimation of Gliclazide and Quercetin in Formulation

Abstract: A simple, quick, accurate, precise, and cost-effective spectrophotometric approach for simultaneous estimation of Gliclazide and Quercetin has been developed by Simultaneous equation method. Gliclazide and Quercetin shows absorbance maximum at 226 and 255 nm respectively, as a result absorbance was measured at these wavelengths for the estimation of Gliclazide and Quercetin. In concentration ranges from 2-18 g/ml, and 2-22 g/ml for Gliclazide and Quercetin respectively followed Beer-Lambert's law. The method has been developed and validated in accordance with ICH recommendations and can be used for estimation of Gliclazide and Quercetin in formulations.