High-speed cell rotation based on a vibration-induced flow designed by evaluations of vertical flow around microstructures

The techniques of cell rotation benefit various fields. We have proposed a cell rotation method based on a vibration-induced flow. Vibration-induced flow is a localized flow generated when vibrations are applied to microstructures. Proposed method uses a vortex flow generated by the flow around three micropillars. This method also enables to control angular velocity by changing voltages corresponding to amplitudes of vibrations. However, there is a problem that cells stop rotating due to floating when applying high voltage for high-speed cell rotation. Therefore, we aim to design the micropillars that enables high-speed cell rotation. In this study, we analyzed a flow causing cell floating with the various heights of the micropillars. Furthermore, we evaluated rotation of mouse oocytes using the micropillars of different heights. We succeeded in high-speed rotation of mouse oocytes at approximately 802 degrees/s by using low-height structures.