Bioevaluation of Cascabela thevatica plant extract

The In vitro antibacterial activity of Cascabela thevatica plant extract has been investigated against Escherichia coli, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae using the agar disc diffusion method. The alcoholic extract was found effective against Escherichia coli, Staphylococcus aureus and Salmonella typhi but does't show any activity against lebsiella pneumoniae. The antibacterial activity is attributed to the presence of alkaloids, which was confirmed by gas liquid chromatography (GLC) and positive alkaloid test. The minimum inhibitory concentration (MIC) was determined by paper disc diffusion method and the results were compared with reference antibiotic tetracycline (one unit solution).