Effect of cytokinin and gibberellic acid applications on seed germination and growth of Rauvolfia verticillata plant

Abstract. Lestari S, Solichatun, Anggarwulan E. 2018. Effect of cytokinin and gibberellic acid applications on seed germination and growth of Rauvolfia verticillata plant. Cell Biol Dev 2: 27-32. The purposes of this research are to study the effect of cytokinin and gibberellic acid (GA3) on seed germination and growth of Rauvolfia verticillata Lour. or pule pandak and to determine the best treatment combination that has an optimal effect on the seed germination and growth of R. verticillata. This study was done in a complete randomized design by combination treatment of gibberellic acid and cytokinin that consist of 6 treatment combinations including GA3 0 ppm/ cytokinin 0 ppm, GA3 50 ppm/ cytokinin 0 ppm, GA3 0 ppm/ cytokinin 50 ppm, GA3 50 ppm/ cytokinin 50 ppm, GA3 50 ppm/ cytokinin 75 ppm, GA3 75 ppm/ cytokinin 50 ppm and 8 replicates. The treatments were given by soaking the mature seed in the hormone solution. Some parameters such as germination and growth parameter were measured. The result shows that presoaking treatment with GA3 50 ppm had a greater influence on germinating time, germination percentage, plant height, leaf number, leaf wide, and dry weight than presoaking use cytokinin 50 ppm. Presoaking treatment using a combination of GA3 and cytokinin (G50S50) has a significant influence on increasing fresh weight and dry weight. The combination of GA3 and cytokinin in the concentration of G50S75 influences fresh weight and dry weight plants. The combination of GA3 and cytokinin in concentration G75S50 influences increased germination percentage, plant height, leaf number, and leaf wide.