Ranunculus sceleratus as a Model Species to Decrypt the Role of Ethylene in Plant Adaptation to Salinity

The aim of the present study was to develop an experimental system for an exploration of ethylene-dependent responses using intact growing Ranunculus sceleratus plants and to approbate the system for assessing the role of ethylene in salinity tolerance and ion accumulation. Plants were cultivated in sealed plastic containers in a modified gaseous atmosphere by introducing ethylene or 1-methylcyclopropene (1-MCP), a competitive inhibitor of ethylene action. High humidity inside the containers induced a fast elongation of the leaf petioles of R. sceleratus. The effect was ethylene-dependent, as 1-MCP completely blocked it, but exogenous ethylene further promoted petiole elongation. Exogenous ethylene decreased (by 48%) but 1-MCP increased (by 48%) the Na+ accumulation in leaf blades of NaCl-treated plants. The experimental system was further calibrated with ethylene and silica xerogel, and the optimum concentrations were found for inducing leaf petiole elongation (10 μL L–1 ethylene) and preventing leaf petiole elongation (200 g silica xerogel per 24 L), respectively. The second experiment involved a treatment with NaCl in the presence of 1-MCP, ethylene, or 1-MCP + ethylene, both in normal and high air humidity conditions. In high humidity conditions, NaCl inhibited petiole elongation by 25% and ethylene treatment fully reversed this inhibition and stimulated elongation by 12% in comparison to the response of the control plants. Treatment with 1-MCP fully prevented this ethylene effect. In normal humidity conditions, NaCl inhibited petiole elongation by 20%, which was reversed by ethylene without additional elongation stimulation. However, 1-MCP only partially inhibited the ethylene effect on petiole elongation. In high humidity conditions, ethylene inhibited Na+ accumulation in NaCl-treated plants by 14%, but 1-MCP reversed this effect. In conclusion, the stimulation of endogenous ethylene production in R. sceleratus plants at a high air humidity or in flooded conditions reverses the inhibitory effect of salinity on plant growth and concomitantly inhibits the accumulation of Na+ in tissues. R. sceleratus is a highly promising model species for use in studies regarding ethylene-dependent salinity responses and ion accumulation potential involving the manipulation of a gaseous environment.