A Heterologous Principle in Human Cancerous Liver: Its Effect Upon the Brown-Pearce Tumor

Summary A method for obtaining a heterologous acetone-soluble fraction from human cancerous tissue is described. This fraction coupled with a protein was employed to obtain an antiserum by injection into rabbits. Both the acetone-soluble fraction and its antiserum were subjected to experimental procedure by injection into rabbits previously inoculated intraocularly with Brown-Pearce carcinoma. Two types of controls, both with intraocular implants, were used: (1) noninjected animals and (2) animals injected with normal antigen obtained from human non-cancerous tissue and with normal rabbit serum. The tumor growth was not modified by injection with normal rabbit serum and acetone-soluble, protein-coupled fraction obtained from human non-cancerous tissue. Inhibition of tumor growth was demonstrable in those animals inoculated with a rapidly growing Brown-Pearce tumor and injected with a heterologous acetone-soluble lipoid fraction obtained from human cancerous tissue and with its antiserum.