UV-Vis Spectroscopic Characterization of β-Cyclodextrin-Vanillin Inclusion Complex

Cyclodextrin molecules can form inclusion complexes with various compounds of appropriate shape and size. The complexation can enhance the solubility and stability of the inclusion guest compound. The stoichiometry and stability constant of the host-guest complex are highly important for physical, chemical, biological, and environmental studies. A simple and rapid spectroscopic method investigated the inclusion of vanillin and β-cyclodextrin (β-CD). The continuous variation technique was used to estimate the stoichiometry of the inclusion complex. The association constant of vanillin with β-CD was determined by using Benesi- Hildebrand and Scott's methods which were calculated to be 179 and 187 M-1, respectively, with the stoichiometry ratio, was 1:1 for the inclusion complex of β-CD with vanillin.