The Effect of Temperature and Concentration on The Brucella Antigen Activity Used for Diagnosis of Brucellosis by Tube Agglutination Test

The current study handled the preparation of a tube agglutination test antigen from a record culture Brucella Abortus S99 according to the European method and at different concentrations (1%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% and 5%), by adding additional microbial cells, these concentrations were compensated by different temperatures (60, 70, 80, 95 and 1100C), to study the effect of the concentration and temperature on the activity of the tube agglutination test antigen in comparison with the antigen prepared by bio- Merieux.