Динамика содержания фитогормонов в каллусной ткани при индукции органогенеза in vitro зародышей Picea abies [L. ] Karst

The content of IAA, ABA, cytokinins (zeatin), 2iP-N6, IPA in callus tissues during the induction of organogenesis in embryos of Norway spruce is researched. Organogenesis was induced on a medium containing thiduazuron and IAA. The main phytohormone inducing differentiation of adventitious buds is cytokinin of the zeatin group in the tissues. Embryos capable of organogenesis had increased levels of zeatin as compared to embryos incapable of organogenesis. Isolated embryos acquired the ability for organogenesis when the level of zeatin and zeatin-ribozida reached 8610 ng/g dry weight and remained above this level throughout the passage. The process of callus formation is greatly influenced by the content of IAA and ABA in the cultured tissues, but a significant increase in the content of these groups of phytohormones resulted in inhibition of both callus induction and organogenesis.