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2022•NanoscaleOpen access

Role of detergents and nuclease inhibitors in the extraction of RNA from eukaryotic cells in complex matrices

Cian Holohan, Nathan Feely, Peng Li, Gerard Curran, Gil U. Lee

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Abstract

serum albumin. The qRT-PCR sensitivity was found to decrease as the concentration of this protein was increased; however, this was linked to an increased RNase activity and not the concentration of the protein itself. These results lead us to recommend a reformulation of LB for clinical samples, and to conclude that sensitive qRT-PCR RNA analysis can be performed in serum with the timely addition of an RNase inhibitor.

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serum albumin. The qRT-PCR sensitivity was found to decrease as the concentration of this protein was increased; however, this was linked to an increased RNase activity and not the concentration of the protein itself. These results lead us to recommend a reformulation of LB for clinical samples, and to conclude that sensitive qRT-PCR RNA analysis can be performed in serum with the timely addition of an RNase inhibitor.

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Available abstract

serum albumin. The qRT-PCR sensitivity was found to decrease as the concentration of this protein was increased; however, this was linked to an increased RNase activity and not the concentration of the protein itself. These results lead us to recommend a reformulation of LB for clinical samples, and to conclude that sensitive qRT-PCR RNA analysis can be performed in serum with the timely addition of an RNase inhibitor.

Key concepts: Chaotropic agent, Lysis, Pulmonary surfactant, Extraction (chemistry), Nuclease, Lysis buffer, RNA, Cell

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Role of detergents and nuclease inhibitors in the extraction of RNA from eukaryotic cells in complex matrices — Research Paper | ScholarLens