2020•Unpublished venueOpen access
Masson's Trichrome Staining Protocol for FFPE spheroids and microtissues v1
Angela Denn, Zbigniew Mikulski
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Abstract
The following protocol has been optimized for image analysis-based quantification of collagen within FFPE spheroid samples. It is run on a Leica ST5020 automated stainer. This protocol can be adapted to manual staining. PURPOSE: Trichrome stains are used to differentiate collagen from other tissues. They are usually nuclear and cytoplasmic stains, followed by differentiation, then followed by a collagen fiber stain. Trichrome stains are useful for indicating fibrotic changes. PRINCIPLE: The staining reaction is based on the differential effect of acid dye on collagen and other tissue components.