Antituberculosis agents of Lactobacillus plantarum and Pediococcus acidilactici Lactic Acid Bacteria in Breast milk isolates against Mycobacterium tuberculosis

Tuberculosis (TB) is one of the infectious diseases that have become a major problem in Indonesia. This disease is caused by the Mycobacterium tuberculosis bacteria. The bacteria are commonly treated with antibiotics. However, the use of irrelevant antibiotics is the most influential factor of antibiotic resistance. Therefore, natural ingredients are required as novel antibiotic agents, some of which are sourced from lactic acid bacteria. This study aims at investigating the antituberculosis activities of Pediococcus acidilactici and Lactobacillus plantarum isolated from breast milk against M. tuberculosis. Pediococcus acidilactici and Lactobacillus plantarum breast milk isolates were rejuvenated with MRS (De Man, Rogosa and Sharpe) broth media, and the supernatant was then neutralized to pH 7.0 using a pH meter by adding 1N NaOH solution. Antituberculosis activity test was performed with Lowensten Jensen media to investigate the growth of M. tuberculosis. The results of this study showed that Pediococcus acidilactici had antituberculosis activity at 48 hours at concentrations of 80% and 90%, while the secondary metabolites of L. plantarum had antituberculosis activity at 24 hours and 48 hours at concentrations of 60%, 70%, 80%, 90%. Therefore, this study concludes that P. acidilactici and L. plantarum bacteria have the potential to be developed as antituberculosis agents.