Fractal analysis of anatomical structures linear contours: modified Caliper method vs Box counting method

Fractal analysis estimates the metric dimension and complexity of the spatial configuration of different anatomical structures. This allows the use of this mathematical method for morphometry in morphology and clinical medicine. Two methods of fractal analysis are most often used for fractal analysis of linear fractal objects: the Box counting method (Grid method) and the Caliper method (Richardson’s method, Perimeter stepping method, Ruler method, Divider dimension, Compass dimension, Yard stick method). The aim of the research is a comparative analysis of two methods of fractal analysis – Box counting method and author's modification of Caliper method for fractal analysis of linear contours of anatomical structures. A fractal analysis of three linear fractals was performed: an artificial fractal – a Koch snowflake and two natural fractals – the outer contours of the pial surface of the human cerebellar vermis cortex and the cortex of the cerebral hemispheres. Fractal analysis was performed using the Box counting method and the author's modification of the Caliper method. The values of the fractal dimension of the artificial linear fractal (Koch snowflakes) obtained by the Caliper method coincide with the true value of the fractal dimension of this fractal, but the values of the fractal dimension obtained by the Box counting method do not match the true value of the fractal dimension. Therefore, fractal analysis of linear fractals using the Caliper method allows you to get more accurate results than the Box counting method. The values of the fractal dimension of artificial and natural fractals, calculated using the Box counting method, decrease with increasing image size and resolution; when using the Caliper method, fractal dimension values do not depend on these image parameters. The values of the fractal dimension of linear fractals, calculated using the Box counting method, increase with increasing width of the linear contour; the values calculated using the Caliper method do not depend on the contour line width. Thus, for the fractal analysis of linear fractals, preference should be given to the Caliper method and its modifications.