1997•Journal of Surgical OncologyRequires access
The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase‐1) in the invasion of gastric cancer
Yoshihiko Sakurai, Yoshihide Otani, Kaori Kameyama, Naoki Igarashi, Tetsuro Kubota, Koichiro Kumai, Masaki Kitajima
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Abstract
Background and Objectives In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase-1: MMP-1) has been also investigated in relation to cancer invasion and metastasis. Methods We investigated the formation and mechanism of MMP-1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results Production of MMP-1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN-74. Localization of MMP-1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions These results suggested that the production of MMP-1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells. J. Surg. Oncol. 1997;66:168–172. © 1997 Wiley-Liss, Inc.