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1996•FEMS Microbiology LettersOpen access

High frequency gene conversion among benomyl resistant transformants in the entomopathogenic fungus Metarhizium anisopliae

M Bogo

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Abstract

Three different methods, (i) PEG, (ii) electroporation and (iii) biolistic, were employed to transform Metarhizium anisopliue using benomyl resistance as a selectable marker.Transformation frequencies and mitotic stability varied for each method, from 0.8 to 6.9 transformants pg-' of DNA and 46%, respectively, by the PEG method; 1.3 to 1.8 transformants pgLg-l of DNA and 67% by electroporation;and 32 to 201 transformants pg -' of DNA and 90% by biolistic.We demonstrate by PCR that 60% of the transformants were generated by gene conversion.

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Three different methods, (i) PEG, (ii) electroporation and (iii) biolistic, were employed to transform Metarhizium anisopliue using benomyl resistance as a selectable marker.Transformation frequencies and mitotic stability varied for each method, from 0.8 to 6.9 transformants pg-' of DNA and 46%, respectively, by the PEG method; 1.3 to 1.8 transformants pgLg-l of DNA and 67% by electroporation;and 32 to 201 transformants pg -' of DNA and 90% by biolistic.We demonstrate by PCR that 60% of the transformants were generated by gene conversion.

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Available abstract

Three different methods, (i) PEG, (ii) electroporation and (iii) biolistic, were employed to transform Metarhizium anisopliue using benomyl resistance as a selectable marker.Transformation frequencies and mitotic stability varied for each method, from 0.8 to 6.9 transformants pg-' of DNA and 46%, respectively, by the PEG method; 1.3 to 1.8 transformants pgLg-l of DNA and 67% by electroporation;and 32 to 201 transformants pg -' of DNA and 90% by biolistic.We demonstrate by PCR that 60% of the transformants were generated by gene conversion.

Key concepts: Metarhizium anisopliae, Entomopathogenic fungus, Benomyl, Entomopathogenic fungi, Fungus, Biology, Metarhizium, Microbiology

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High frequency gene conversion among benomyl resistant transformants in the entomopathogenic fungus Metarhizium anisopliae — Research Paper | ScholarLens