Capillary Isotachophoresis
F.M. Everaerts, Th.P.E.M. Verheggen
Abstract
F.M. Everaerts, Th.P.E.M. Verheggen
Abstract
Isotachophoresis (displacement electrophoresis) belongs to one of the four basic electrophoretic separation techniques: Moving boundary electrophoresis, zone electrophoresis and isoelectric focusing. In the article presented capillary isotachophoresis is described. Basic principles are outlined and attention is given to qualitative and quantitative evaluation of isotachophoretic analysis in which conductimetric, potential gradient, photometric (UV-absorption and fluorimetric) detectors are used. Separation of anions and cations is shown and a way for automatic signal processing is given.
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Isotachophoresis (displacement electrophoresis) belongs to one of the four basic electrophoretic separation techniques: Moving boundary electrophoresis, zone electrophoresis and isoelectric focusing. In the article presented capillary isotachophoresis is described. Basic principles are outlined and attention is given to qualitative and quantitative evaluation of isotachophoretic analysis in which conductimetric, potential gradient, photometric (UV-absorption and fluorimetric) detectors are used. Separation of anions and cations is shown and a way for automatic signal processing is given.
Key concepts: Isotachophoresis, Capillary electrophoresis, Chromatography, Electrophoresis, Isoelectric focusing, Chemistry, Capillary action, Analytical Chemistry (journal)