Cell Cycle Synchronization

Abstract Cell synchronization methods have provided effective tools in cell cycle research along with the development in control of proliferative diseases andin vitrocell growth. Several chemically induced synchronization methods, for accumulating and isolating cells in the various phases of the cell cycle have been developed for adherent and non‐adherent cells, and although widely accepted they have major drawbacks. Physical methods such as centrifugal elutriation do not perturb cell cycle progression and produce highly synchronized viable cell populations. In this article, conventional and recent developments of methods for cell synchronization for various cell types and for monitoring and measurement of cell cycle and cell cycle phase duration are described.