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2016•ASM Press eBooksRequires access

Agar Dilution MIC Test

Amy L. Leber

Open publisher page 2 citations

Abstract

The agar dilution MIC test is a quantitative method for the determination of in vitro activity of an antimicrobial agent against bacterial isolates. A series of agar plates, each containing a unique concentration of an antimicrobial agent, is inoculated with up to 36 isolates per plate. Following incubation using conditions appropriate for the test organisms, the MIC is determined by observing the lowest concentration of antimicrobial agent that inhibits visible growth of each test isolate. MICs obtained are interpreted as susceptible, intermediate, resistant, or nonsusceptible based on criteria defined by the CLSI (1, 2).

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What this paper is about

The agar dilution MIC test is a quantitative method for the determination of in vitro activity of an antimicrobial agent against bacterial isolates. A series of agar plates, each containing a unique concentration of an antimicrobial agent, is inoculated with up to 36 isolates per plate. Following incubation using conditions appropriate for the test organisms, the MIC is determined by observing the lowest concentration of antimicrobial agent that inhibits visible growth of each test isolate. MICs obtained are interpreted as susceptible, intermediate, resistant, or nonsusceptible based on criteria defined by the CLSI (1, 2).

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Available abstract

The agar dilution MIC test is a quantitative method for the determination of in vitro activity of an antimicrobial agent against bacterial isolates. A series of agar plates, each containing a unique concentration of an antimicrobial agent, is inoculated with up to 36 isolates per plate. Following incubation using conditions appropriate for the test organisms, the MIC is determined by observing the lowest concentration of antimicrobial agent that inhibits visible growth of each test isolate. MICs obtained are interpreted as susceptible, intermediate, resistant, or nonsusceptible based on criteria defined by the CLSI (1, 2).

Key concepts: Agar Dilution Method, Agar dilution, Antimicrobial, Agar, Dilution, Minimum inhibitory concentration, Microbiology, Agar plate

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